Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Involvement of long non-coding RNA (lncRNA) MALAT1 in shear stress regulated adipocyte differentiation

doi: 10.3389/fbioe.2025.1570518

Figure Lengend Snippet: Overview of experimental design. (A) Experimental design. (B) Schematic illustration of endocytic uptake of LNA nanobiosensor by MSCs for lncRNA detection. Once internalized, the LNA detecting probe is displaced from the dsLNA complex and binds to the target lncRNA, allowing the fluorophore to fluorescence. (C) Representative fluorescence image of lncRNA MALAT1 expression in MSCs. Scale bar: 50 µm. (D) Simulated distribution of orbital shear stress. Yellow labeled rectangles indicate the location of well-plates. The estimated shear stress was in the range of 0.3–0.6 Pa.

Article Snippet: To silence lncRNA MALAT1 expression, MSCs were seeded in 12-well plates and transfected with MALAT1 antisense probe (Qiagen) at a concentration of 50 nM (final concentration) using Lipofectamine 2000 (Invitrogen) transfection reagent following manufacturer’s instructions.

Techniques: Fluorescence, Expressing, Shear, Labeling

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Involvement of long non-coding RNA (lncRNA) MALAT1 in shear stress regulated adipocyte differentiation

doi: 10.3389/fbioe.2025.1570518

Figure Lengend Snippet: Shear stress modulates the expression of MALAT1. (A) Representative bright field and fluorescence images of MSCs in control and shear conditions with adipogenic induction medium (5 days), respectively. The green fluorescence signal shows MALAT1 expression. Red: Phalloidin (F-actin), blue: Hoechst 33342 (Nucleus). Scale bar: 100 µm. (B) Comparison of mean fluorescence intensity of MALAT1 expression for MSCs cultured in adipogenic induction medium. (C) Comparison of mean fluorescence intensity of MALAT1 expression for MSCs cultured in basal medium. Data are expressed as mean ± s.e.m. (n = 3). A two-tailed t-test was used to analyze differences between control and shear conditions. *, p < 0.05; **, p < 0.01; ***, p < 0.005.

Article Snippet: To silence lncRNA MALAT1 expression, MSCs were seeded in 12-well plates and transfected with MALAT1 antisense probe (Qiagen) at a concentration of 50 nM (final concentration) using Lipofectamine 2000 (Invitrogen) transfection reagent following manufacturer’s instructions.

Techniques: Shear, Expressing, Fluorescence, Control, Comparison, Cell Culture, Two Tailed Test

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Involvement of long non-coding RNA (lncRNA) MALAT1 in shear stress regulated adipocyte differentiation

doi: 10.3389/fbioe.2025.1570518

Figure Lengend Snippet: lncRNA MALAT1 knockdown inhibits adipocyte differentiation. (A) Representative images of differentiated MSCs in different conditions (static and shear) after 5 and 10 days of induction. Scale bar: 100 µm. (B) Comparison of the number of differentiated cells after MALAT1 silencing for both cells under static and shear conditions (n = 3). Data are expressed as mean ± s.e.m. (n = 3). One-way analysis of variance (ANOVA) was used for multiple group comparisons. A two-tailed t-test was used to analyze differences between control and shear conditions. *, p < 0.05; **, p < 0.01; ***, p < 0.005.

Article Snippet: To silence lncRNA MALAT1 expression, MSCs were seeded in 12-well plates and transfected with MALAT1 antisense probe (Qiagen) at a concentration of 50 nM (final concentration) using Lipofectamine 2000 (Invitrogen) transfection reagent following manufacturer’s instructions.

Techniques: Knockdown, Shear, Comparison, Two Tailed Test, Control